CDK5 Genotyping by PCR

Primers:

Cdk5f1 (5’ to 3’): ATT GTG GCT CTG AAG CGT GTC
Cdk5r1 (5’ to 3’): CTT GTC ACT ATG CAG GAC ATC
Pgk1 (5’ to 3’): TCC ATC TGC ACG AGA CTA GT

PCR reaction mixture:

PCR cycles:

Run PCR products on 1% Agarose gel and determine genotypes: